one-step approach

Additionally, one-step approach is reported to be less accurate compared to the two-step approach.

The one-step approach is thought to minimize experimental variation by containing all of the enzymatic reactions in a single environment.

In the one-step approach, the entire reaction from cDNA synthesis to PCR amplification occurs in a single tube.

Unlike conventional inversion and geomodeling algorithms, geostatistical inversion takes a one-step approach, solving for impedance and discrete property types or lithofacies at the same time.

However, the starting RNA templates are prone to degradation in the one-step approach, and the use of this approach is not recommended when repeated assays from the same sample is required.