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The midline nuclei third order neurons then synapse to the cingulate and insular cortex.
The affective/motivational information is mediated by connections to reticular formation, midbrain, and the midline nuclei of the thalamus.
Cell group B1 occupies the midline nucleus raphes pallidus and adjacent structures in the caudal medulla oblongata of the rodent and the primate.
Some definitions of the VTA also include the midline nuclei (i.e. the interfascicular nucleus, rostral linear nucleus, and central linear nucleus).