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A throat culture taken on the day of microbiological diagnosis was negative.
No definite microbiological diagnoses could be made for the remaining 74 (52%) patients.
Final microbiological diagnosis was made by two infectious disease specialists who weighed all available clinical evidence.
The microbiological diagnosis was reached by using conventional cultures with specific biochemical tests and eubacterial PCR amplification followed by direct sequencing.
This involves, first, the collection of comprehensive data on infectious diseases, using precise methods, taking into account not only clinical diagnosis, but also microbiological diagnosis, as far as possible.
Analysis of survival by microbiological diagnosis was done with total events since the small number of definite diagnoses in each patient category precluded the use of the Kaplan-Meier approach.
IGRAs cannot distinguish between latent infection and active tuberculosis (TB) disease, and should not be used for diagnosis of active TB, which is a microbiological diagnosis.