metaphase chromosome

The end result is the metaphase chromosome.

Then, an interphase or metaphase chromosome preparation is produced.

In addition metaphase chromosome spreads prepared from SiHa cells have been examined.

The analysis of metaphase chromosomes is one of the main tools of classical cytogenetics and cancer studies.

Metaphase chromosomes make the classical picture of chromosomes (karyotype).

For each FISH experiment at least 20 independent metaphase chromosome preparations were examined.

Newer array technology will not detect the ring chromosome and the standard metaphase chromosome analysis has been recommended.

FISH can either be performed as a direct approach to metaphase chromosomes or interphase nuclei.

"Nucleosomes in metaphase chromosomes."

However, a trained technologist is required to distinguish subtle differences in banding patterns on bent and twisted metaphase chromosomes.

Human metaphase chromosome spreads were obtained from PHA-stimulated normal lymphocytes using standard procedures (28).

The metaphase chromosomes are treated with trypsin (to partially digest the chromosome) and stained with Giemsa.

In flow karotyping, isolated metaphase chromosomes are stained and measured in a flow microfluorometer.

It localises to euchromatin within interphase nuclei but is enriched within the centromeric heterochromatin of metaphase chromosomes.

Higher-level DNA packaging of the 30 nm fibre into the metaphase chromosome (during mitosis and meiosis).

Because of the limited resolution of metaphase chromosomes, aberrations smaller than 5-10 Mb cannot be detected using conventional CGH.

Routine chromosome analysis refers to analysis of metaphase chromosomes which have been banded using trypsin followed by Giemsa, Leishmanns, or a mixture of the two.

The vastly superior and more rapid method for metaphase chromosome preparation, of air-drying of germ cells following a short hypotonic pre-treatment, has long since superseded squashing.

In array CGH, the metaphase chromosomes are replaced by cloned DNA fragments (+100-200 kb) of which the exact chromosomal location is known.

Integration of HPV16 in metaphase chromosome spreads prepared from the SiHa cell-line has also been demonstrated using a fluorescent reporter molecule (Figures 1Eand 1F).

The probe mixtures were then applied to rat fibroblast metaphase chromosome slides (previously denatured at 73 C for 2 min in 70 % formamide, 2 x SSC).

In this method, Flow-FISH uses interphase cells rather than metaphase chromosomes and hybridizes the PNA probes in suspension.

A traditional exam involving metaphase chromosome analysis is often unable to identify features that distinguish one disease from another, due to subtle chromosomal features; FISH can elucidate these differences.

Biotinylated probe DNAs were localised on R-banded normal male metaphase chromosomes by FISH according to procedures described elsewhere (Leversha et al., in preparation).

Because prophase and prometaphase chromosomes are more extended than metaphase chromosomes, the number of bands observable for all chromosomes increases from about 300 to 450 to as many as 800.