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Klein's group found populations with metacentric chromosomes in different regions of Europe, but concentrated its effort on the system of metacentrics in southern Germany.
The domestic horse genome includes one metacentric chromosome that is homologous to two acrocentric chromosomes in the conspecific but undomesticated Przewalski's horse.
The Y is a small metacentric chromosome, while the X is a large metacentric chromosome.
In some cases, a metacentric chromosome is formed by balanced translocation: the fusion of two acrocentric chromosomes to form one metacentric chromosome.
Ten non homologous acrocentric chromosomes in one animal correspond to five metacentric chromosomes in the other; the other eight pairs of metacentrics are common to all forms.
This may reflect either fixation of a balanced Robertsonian translocation in domestic horses or, conversely, fixation of the fission of one metacentric chromosome into two acrocentric chromosomes in Przewalski's horses.
A similar situation exists between the human and great ape genomes; in this case, because more species are extant, it is apparent that the evolutionary sequence is a reduction of two acrocentric chromosomes in the great apes to one metacentric chromosome in humans (see Karyotype).