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B cells may have evolved from macrophage-like cells during the formation of the adaptive immune system.
Microglia, another type of glial cell, act as macrophage-like cells in the CNS when activated.
This compound induces mammalian cells (specifically, the cell line HL60) to differentiate into granulocyte- or macrophage-like cells.
Precursor HL-60 cells are spherical and grow in suspension, whereas the differentiated macrophage-like cells become adherent and spread.
Murine macrophage-like cell line (J774A.1) was from American Type Culture Collection (Manassas, VA).
First identified in the conditioned media of human macrophage-like cells, HB-EGF is an 87 amino acid glycoprotein which displays highly regulated gene expression.
They show increased CO production on phagocytosis and can be differentiated into macrophage-like cells using for example phorbol 12-myristate 13-acetate (commonly known as PMA or TPA).
Fibroblasts macrophage-like cells (PU.1 and C/EBPα or C/EBPβ)
Overall, the time course of cellular events following L. monocytogenes infection in Caco-2 cells closely parallels the time course reported for macrophage-like cell lines and other epithelial cell lines [ 16].
MHC class 2 positivity was largely confined to subepithelial clsuters of macrophage-like cells (CD68 + ) with foamy cytoplasm: double labelling of these clusters with anti-IL2R (CD25) confirmed with prescence of activated macrophages.
The tumor promoter phorbol myristate acetate (PMA) has been shown to activate the NADPH (nicotinamide adenine dinucleotide phosphate) oxidase and initiate the oxidative burst response in various phagocytes, including neutrophils and the macrophage-like cell line J774A.1 [ 6 7 ] .