lead citrate

For staining, uranyl acetate and lead citrate can be used.

(xi) Centrifuge the lead citrate solution for 10 min at 5000 rpm.

Uranyl acetate and lead citrate are commonly used to impart contrast to tissue in the electron microscope.

Thin sections were prepared and grids were stained with uranyl acetate and lead citrate.

Typically thin sections are stained for several minutes with an aqueous or alcoholic solution of uranyl acetate followed by aqueous lead citrate.

The samples were post-fixed with 1% glutaraldehyde and stained with uranyl acetate and lead citrate.

All ultrathin sections were double stained with uranyl acetate and Reynolds lead citrate and then observed under a Philips 410 microscope.

One micron sections were cut for orientation; thin sections were stained with uranyl acetate and lead citrate [ 70 ] .

For electron microscopy imaging, ultra-thin sections were stained with uranyl acetate and lead citrate (5 minutes each) and viewed under a Philips CM10 transmission electron microscope.

Semithin and ultrathin sections were cut and contrasted with lead citrate and examined with a JEOL X100 electron microscope.

Thin sections were cut parallel to the substratum, stained with uranyl acetate and lead citrate, and examined with a Jeol 100CXII electron microscope at 80kV.

Thin sections of ventral horn were cut from the Epon tissue blocks, stained with uranyl acetate and lead citrate, and visualized using a Philips CM10 transmission electron microscope.

In each of the 40 H pylori positive cases an ultra-thin section of a selected area was contrasted with uranyl acetate lead citrate and studied with a Hitachi H-800 electron microscope.

(b) Lead citrate: add 1.33 g of lead nitrate and 1.76 g of sodium citrate to 30 ml of CO2-free double-distilled water in a 50 ml volumetric flask.

Specimens were fixed in half-strength Karnovsky fixative, postfixed in osmium tetroxide, embedded in Araldite, thin sectioned and stained with uranyl acetate and lead citrate in the usual way.

The ultrathin sections are collected on 3mm copper (mesh) grids and stained with uranyl acetate and lead citrate to make the contents of the tissue electron dense (and thus visible in the electron microscope).

Sections (1 m) were cut and stained with toluidine blue for observation by light microscopy, followed by ultrathin sections of approximately 100 nm, collected on nickel grids and stained with unranyl acetate and lead citrate, for observation on a Philips CM-10 electron microscope.