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The kjeldahl method is poorly sensitive in the original version.
Kjeldahl method: A measure of nitrogen content in a sample.
The amount of nitrogen in a chemical substance can be determined by the Kjeldahl method.
After conversion under the form of ammonia, the total nitrogen is then determined by Kjeldahl method.
Kjeldahl method for determining total nitrogen.
The classic assays for protein concentration in food are the Kjeldahl method and the Dumas method.
It is also considerably faster than the Kjeldahl method, taking a few minutes per measurement, as compared to the hour or more for Kjeldahl.
The traditional method for measuring total protein uses the biuret reagent, but other chemical methods such as Kjeldahl method, dye-binding and refractometry are now available.
They characterized the product to confirm that it was a N-alkyl amide through the Kjeldahl method, which quantitatively determines nitrogen presence in chemical substances.
The standard tests for crude protein content used in the food industry (Kjeldahl method and Dumas method are used for official purposes) measure total nitrogen.
The Kjeldahl method's universality, precision and reproducibility have made it the internationally-recognized method for estimating the protein content in foods and it is the standard method against which all other methods are judged.
As with the Kjeldahl method, conversion of the concentration of nitrogen in a sample to the crude protein content is performed using conversion factors which depend on the particular amino acid sequence of the measured protein.
An automated instrumental technique has been developed which is capable of rapidly measuring the crude protein concentration of food samples and is beginning to compete with the Kjeldahl method as the standard method of analysis for protein content for some foodstuffs.
The limitations of the Kjeldahl method were at the heart of the Chinese protein export contamination in 2007 and the 2008 Chinese milk scandal in which the industrial chemical melamine was added to the milk or glutens to increase the measured "protein".
Kjeldahl method is not applicable to compounds containing Nitrogen in Nitro and Azo groups and Nitrogen present in the ring (e.g. Pyridine) as Nitrogen of these compounds does not change to Ammonium sulphate under the conditions of this method.