jet injection

When studied, 57 out of 239 people who had received the jet injection tested positive for hepatitis B.

The experiments demonstrated that low-volume jet injection successfully induced immunity in rabbits.

Mean luciferase activity following needle and syringe injection was 50 fold lower than jet injection.

High levels of gene expression were not required for successful intradermal genetic immunization by low-volume jet injection.

Jet injection had different insulin delivery peaks and durations as compared to needle injection.

Moreover, there were no significant differences between jet injection and particle bombardment in the maximal antibody titers or number of injections before response.

In the low-volume jet injection and particle bombardment groups, gene expression levels in the skin did not predict the magnitude of the immune response.

Gene expression levels following particle bombardment were 10-100 fold higher than those found following low-volume jet injection but the immune response was equivalent.

To establish skin penetration depth following low-volume jet injection, one rabbit was injected at 6 different sites with a 1% India ink solution.

Background Jet injection is a relatively simple and low-cost method for gene transfer into somatic tissues [ 1 ] .

While immune responses were comparable, luciferase gene expression levels in the skin following particle bombardment were 10-100 times higher than low-volume jet injection.

Low-volume jet injection deliberately limits the volume of the injected solution and is utilized to target delivery to small tissue areas [ 2 ] .

The experiments demonstrate that intradermal low-volume jet injection of a DNA vaccine can be utilized to elicit immune responses.

For optimization of the jet injection experiments, three different injection volume settings (5-μl, 6-μl and 7-μl) at three different pressures (1,2 and 3-Bar) were tested.

When using low-volume jet injection for intradermal delivery the goal is contain the delivery solution within the epidermis and dermis but optimize gene expression levels.

The purified minicircle can be transferred into the recipient cell by transfection or lipofection and into a differentiated tissue by, for instance, jet injection.

This investigation tested the ability of low-volume jet injection to target delivery of a DNA vaccine to the skin for the purpose of inducing immunity.

Delivery to the oral mucosa by jet injection elicited a higher IgA response than intranasal delivery in mice [ 20 ] .

These experiments show that low-volume jet injection specifically targeted delivery of a DNA solution to the skin and that the injection paths did not reach into the underlying tissue.

To establish optimal injection conditions for low-volume jet injection, two rabbits were injected at a total of 15 sites per rabbit with pHCMVIE1-luciferase and euthanized 48 h later.

The low-volume jet injection method was comparable to the more established technique of particle bombardment and was significantly better than the established technique of needle and syringe injection.

Conclusion Low-volume jet injection is an effective new methodology for intradermal DNA immunization that has advantages over the established techniques of needle and syringe injection and particle bombardment.

Dr. Hingson began epidural and jet injections as a fledgling physician when he was the director of anesthesia at the United States Marine Hospital on Staten Island from 1941 to 1943.

Optimal injection parameters for low-volume jet injection, particle bombardment and needle and syringe injection Optimal injection parameters for each technique were established by comparing relative mean expression levels of the luciferase reporter gene in the skin 48 hours after injection.

Optimization of low-volume jet injection, particle bombardment and needle and syringe injection conditions Low-volume jet injection was performed with a prototype low-volume jet injector with adjustable injection volume and pressure.