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Two factors determine that immunoelectrophoretic methods are not widely used.
The immunoelectrophoretic analysis ad modum Grabar is the classical method of immunoelectrophoresis.
I. Immunoelectrophoretic analysis of blood serum, collostrum and milk of rabbits and sheep.
The sensitivity and resolving power of crossed immunoelectrophoresis is than that of the classical immunoelectrophoretic analysis and there are multiple variations of the technique useful for various purposes.
The introduction of the immunoelectrophoretic analysis gave a great boost to protein chemistry, some of the very first results were the resolution of proteins in biological fluids and biological extracts.
Godzinska H, Osinska M, Slopek S. 1970 Immunoelectrophoretic studies on blood serum, collostrum and milk of animals of different species in their ontogenetic development.
Brzuchowska W, Godzinska H, Grzybek-Hryncewicz K, Slopek S. 1964 An attempt to identify the opsonizing factor in guinea pig serum with the immunoelectrophoretic method.
In addition proteins are separated by gel electrophoresis on the basis of their apparent molecular weight, which is not accomplished by immunoelectrophoresis, but nevertheless immunoelectrophoretic methods are still useful when non-reducing conditions are needed.