horseradish peroxidase

Horseradish peroxidase has an accessible active site, and many compounds can reach the site of the reaction.

"This is horseradish peroxidase, an enzyme bound to an antibody," Bono explained.

For example, horseradish peroxidase can use a variety of organic compounds as electron donors and acceptors.

Horseradish peroxidase catalyses the oxidation of luminol to 3-aminophthalate via several intermediates.

A secondary anti-human antibody conjugated to an enzyme such as horseradish peroxidase.

Like horseradish peroxidase, lanthanum is used as an electron-dense tracer in molecular biology.

For example, phenols, which are important pollutants, can be removed by enzyme-catalyzed polymerization using horseradish peroxidase.

The enzyme horseradish peroxidase along with hydrogen peroxide has been found to biodegrade carbon nanotubes.

Moreover, "In recent years the technique of marking neurons with the enzyme horseradish peroxidase has become a major tool.

Horseradish peroxidase is a 44,173.9-dalton glycoprotein with 6 lysine residues which can be conjugated to a labeled molecule.

Streptavidin is widely used in Western blotting and immunoassays conjugated to some reporter molecule, such as horseradish peroxidase.

The antibody can either be conjugated to a fluorophore or to horseradish peroxidase for imaging and/or quantification.

After further washing, they were finally incubated with the PAP complex (horseradish peroxidase anti-peroxidase) for 30 minutes.

Amersham International has launched a new range of sensitive, ready to use substrates for horseradish peroxidase and alkaline phosphatase based western blotting detection systems.

Ceccarelli et al. studied frog neuromuscular junctions, stimulating them with markers such as horseradish peroxidase to identify endocytosed organelles.

The secondary antibody is usually linked to biotin or to a reporter enzyme such as alkaline phosphatase or horseradish peroxidase.

The enzyme horseradish peroxidase (HRP), found in the plant, is used extensively in molecular biology and biochemistry.

Horseradish peroxidase is also capable of oxidizing these substrates, but its heme is not covalently bound and becomes damaged during turnover.

Numerous substrates for the horseradish peroxidase enzyme have been described and commercialized to exploit the desirable features of HRP.

The potential for nanoreactors can be demonstrated by binding the enzyme horseradish peroxidase into the membrane itself; trapping the enzyme glucose oxidase.

Horseradish peroxidase is ideal in many respects for these applications because it is smaller, more stable, and less expensive than other popular alternatives such as alkaline phosphatase.

As previously described, enzymes such as horseradish peroxidase or alkaline phosphatase are commonly used to catalyse reactions that give a coloured or chemiluminescent product.

TMB can act as a proton donor for the reduction of hydrogen peroxide to water by peroxidase enzymes such as horseradish peroxidase.

Horseradish peroxidase (HRP) labeled streptavidin was from Zymed, South San Francisco, CA.

The membrane was washed in the same solution and then incubated with a horseradish peroxidase conjugated secondary antibody (1:5000) in the same solution for 30 min.