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A number of subsequent reports (from as far back as the early 1980s), showed that cell suspension (using a shaking-incubator) significantly increased culture growth.

The cell suspension was frozen using liquid nitrogen and rapidly thawed in a 37 C water bath.

Using the same rotor/chamber configuration, large commercial machines are available to process many liters of cell suspension.

(iii) Centrifuge the cell suspension obtained at 50 g for 5 min.

Take up the cell suspension in a 1 ml syringe without a needle.

This can be done on tissue section (fresh or fixed tissue), cell suspension, etc.

This cell suspension was the basis for all the experiments.

Place a few drop of the cell suspension onto a microscope slide and let air dry overnight.

The cell suspension is then dropped onto specimen slides.

They are adequate for cell suspensions and attachment dependent cell types.