The combination of doubled haploidy and molecular marker provides the short cut.

Another disadvantage associated with the double haploidy is the cost involved in establishing tissue culture and growth facilities.

The number of species amenable to doubled haploidy has reached a staggering 250 in just a few decades.

Combined with doubled haploidy it becomes more effective.

Hence one has to take several factors into consideration before deploying doubled haploidy in breeding programmes.

See also main article on Doubled haploidy.

The over-usage of doubled haploidy may reduce genetic variation in breeding germplasm.

Conventional inbreeding procedures take six generations to achieve approximately complete homozygosity, whereas doubled haploidy achieves it in one generation.

Hagerup, O. (1945) Facultative parthenogenesis and haploidy in Epipactis latifolia.

Chen et al. (1994) used marker assisted backcross conversion with doubled haploidy of BC1 individuals to select stripe rust resistant lines in barley.

Here, backcrossing is carried out until a desired level of recombination has occurred and genetic markers are used to detect desired recombinant chromosome substitution lines in the target region, which can be fixed by doubled haploidy.

More specific benefits include the possibility of seed propagation as an alternative to vegetative multiplication in ornamentals, and in species such as trees in which long life cycles and inbreeding depression preclude traditional breeding methods, doubled haploidy provides new alternatives.

Of note, on the SJCRH study 33 of 498 (6.6%) patients in first remission with high-risk features (including 26 with high MRD, six with Philadelphia chromosome-positive ALL, and one with near haploidy) received an allogeneic stem cell transplant, which included total body irradiation.

Studies were conducted comparing DH method and other conventional breeding methods and it was concluded that adoption of doubled haploidy does not lead to any bias of genotypes in populations, and random DHs were even found to be compatible to selected line produced by conventional pedigree method.