glutathione-S-transferase

He has already discovered that variations in the gene for glutathione-S-transferase can affect the age at which the disease starts.

Examples include polyhistidine-tag, glutathione-S-transferase, and maltose binding protein.

To purify, proteins are tagged e.g. using His-tags or GST (glutathione-S-transferase) tags, which can be recognized by a metal ion ligand, such as imidazole.

A glutathione-S-transferase, a detoxification enzyme in parasites isolated from Setaria cervi, a bovine filarial parasite, reduced B. malayi adult parasite burden by more than 82% 90 days post parasite.

Examples of tags in use are the Green Fluorescent Protein (GFP) tag, Glutathione-S-transferase (GST) tag and the FLAG-tag tag.

Examples of E. coli expression vectors are the pGEX series of vectors where glutathione-S-transferase is used as a fusion partner, and the pET series of vectors which uses a T7 promoter.

Recombinant schistosome glutathione-S-transferase, which confers only a partial resistance to infection, induces a potent anti-fecundity immune response that dramatically reduces the number and viability of eggs produced by female parasites, and so reduces the risk of transmission and the pathology associated with infection (A. Capron, Pasteur-Lille).