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After the addition of general transcription factors, reactions were incubated 30 C for 20 min.
This smaller activation probably reflects the absence of general transcription factors in the minimal promoter used.
This box along with the GC box is known for binding general transcription factors.
One surprising result of our work is that there are relatively few recognizable binding sites for known general transcription factors in Drosophila core promoters.
A general transcription factor BTF3 (Fig.
These activators are only fully active in the context of other general transcription factors localized in the proximal region of the promoter.
This allows it to interact with the general transcription factors and RNA polymerase II.
In in vitro experiments it has been shown that H1 is able to bind general transcription factors and specifically repress transcription (8,9).
Core promoters show specificity both in their interactions with enhancers and with sets of general transcription factors that control distinct subsets of genes.
General transcription factor 3C polypeptide 5 is a protein that in humans is encoded by the GTF3C5 gene.
Associated CAK is part of the general transcription factor TFIIH.
The replacement histone H1 of the H1 group, known to interact with general transcription factors, has been found associated with transcriptionally repressed chromatin.
These activator proteins interact with the mediator complex, which recruits polymerase II and the general transcription factors which then begin transcribing the genes.
General Transcription Factor IIF (GTF2F2) is further downstream directed in the same orientation.
In classical silencers, the gene is actively repressed by the silencer element, mostly by interfering with general transcription factor (GTF) assembly.
In eukaryotes, an important class of transcription factors called general transcription factors (GTFs) are necessary for transcription to occur.
Mediator has been shown to associate with general transcription factors, as well as RNA polymerase II, and is essential for activator-dependent transcription.
General transcription factors position RNA polymerase at the start of a protein-coding sequence and then release the polymerase to transcribe the mRNA.
Together with the TATA-binding protein and other TAFs it forms the general transcription factor, TFIID.
TFIIIC is one of the general transcription factors involved in RNA polymerase III-mediated transcription.
It consists of RNA polymerase II, a subset of general transcription factors, and regulatory proteins known as SRB proteins.
NcRNAs also target general transcription factors required for the RNAP II transcription of all genes (Goodrich 2006).
CDK7 is both a CDK-activating kinase (CAK) and a component of the general transcription factor TFIIH.
There were also several sites that appear to be the general transcription factor TFIIB, and both E2F and E2F transcription factor binding sites.
Transcription factor IIB (TFB) is one of several general transcription factors that make up the RNA polymerase II preinitiation complex.