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Fractions can be collected automatically by means of fraction collectors.
Finally, program your fraction collector based on the volumes and/or run times as they appear on the screen.
Systems may also be linked with detectors and fraction collectors providing automation.
As buffer elutes from the column, the samples are collected using a fraction collector.
The venous effluent was collected from the cannulated ileocolic vein with a fraction collector.
The separated proteins are continuously eluted into a physiological eluent and transported to a fraction collector.
Due to variations in time duration required for urine to reach the fraction collectors, peaks of blood and urine are difficult to compare.
The solutions and buffers pass through the column where a fraction collector at the end of the column setup collects the eluted samples.
Common examples are fraction collector racks from LC/MS systems, larger reaction blocks or proprietary sample formats.
A typical laboratory FPLC consist of one or two high-precision pumps, a control unit, a column, a detection system and a fraction collector.
Fraction collector: The fraction collector is typically a rotating rack that can be filled with test tubes or similar containers.
Typical applications include processing HPLC samples in modified fraction collector racks, processing samples for compound storage and high throughput screening or synthesis.
Like the FPLC system, the LPLC system has a spectrophotometer, chart recorder and fraction collector attached to allow you to easily monitor and record the results as they are obtained.
Automated systems will include components normally found on more expensive high performance liquid chromatography (HPLC) systems such as a gradient pump, sample injection ports, a UV detector and a fraction collector to collect the eluent.
As the Joule heat generated during electrophoresis may have a negative impact on the stability and migration behavior of proteins, the separation system, including the electrophoresis chamber and a fraction collector, is cooled in a refrigerator at 4 C (see figure Equipment).