fluorochrome

Once the fluorochrome has been reconstituted, it is essentially irreversible in vitro.

Each of these probes are specific for part of a chromosome, and are labelled with a fluorochrome.

A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation.

The laser illumination that is used to excite the fluorochrome is also used here to destroy the fluorochrome signal.

Fluoro-Jade stain is a fluorochrome derived from fluorescein, and is commonly used in neuroscience disciplines to label degenerating neurons in ex vivo tissue of the central nervous system.

DNA sequencers can be considered optical instruments as they analyse the color and intensity of the light emitted by a fluorochrome attached to a specific nucleotide of a DNA strand.

Anti mouse IgG conjugated to Cy3 fluorochrome (1:250 dilution, 1 h at room temperature) was used to visualize ER antigenicity (Jackson ImmunoResearch, West Grove, PA).

Quantitative PCR is carried out in a thermal cycler with the capacity to illuminate each sample with a beam of light of a specified wavelength and detect the fluorescence emitted by the excited fluorochrome.

The quantity and quality of DNA samples were estimated by comparing band intensities on agarose gel as well as by fluorometry (DyNA Quant 200, Pharmacia) using Hoechst 33258 as the fluorochrome.

Following a wash to remove unbound antibody, the secreted cytokines are visualized by addition of a mixture of Streptavidin labelled with red fluorochrome and antibody to FITC labelled with green fluorochrome.

Cells were then incubated in primary antibody diluted in blocking buffer for at 24 h at 4 C. Following three washes in PBS the cells were incubated with fluorochrome conjugated secondary antisera diluted in blocking buffer (Table 1) for one hour at room temperature.