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He has also developed fluorescent indicators for other bio-relevant ions.
Miyawaki, led us to design novel fluorescent indicators for calcium named cameleons.
It is a triazene dye used as a stain and fluorescent indicator in microscopy.
The Aromatic Analyser replaces the current fluorescent indicator absorption methods.
He also developed fluorescent indicators of calcium ions and other ions important in biological processes.
Practical aspects of measuring [Ca2+] with fluorescent indicators.
Individual chemical Ca fluorescent indicators are utilized for cytosolic calcium measurements in a wide variety of cellular preparations.
GFP) and 2) fluorescent indicators of calcium and other second messengers.
Protein made by the cell according to this DNA information then serves as a fluorescent indicator of calcium concentration.
Quantification of the prevalence of synaptic networks has primarily been through retinal wave detection using Ca fluorescent indicators.
An acidity scale for excited state protonation kinetics in aqueous perchloric acid has been developed using 1-cyanonaph-thalene as a fluorescent indicator.
Vesicle leakage or membrane fusion caused by diltiazem was assessed by the use of vesicles containing fluorescent indicators.
Relative responses from a combination of chemical Ca fluorescent indicators were also used to quantify calcium transients in intracellular organelles such as mitochondria.
Binding of a Ca ion to a fluorescent indicator molecule leads to either an increase in quantum yield of fluorescence or emission/excitation wavelength shift.
All plates are silica gel with UV-254 fluorescent indicator and available with channeling and linear preadsorbent spotting area for faster, more accurate sample application.
We will use isolated heart cells, patch clamp, fluorescent indicators and a sophisticated rapid solution change method (which changes solution bathing a single cell within 100msec) to investigate this.
In another strategy called indicator-displacement assay (IDA) an analyte such as citrate or phosphate ions displace a fluorescent indicator in an indicator-host complex.
If a molecular interaction or a protein conformational change is dependent on ligand binding, this FRET technique is applicable to fluorescent indicators for the ligand detection.
Neurodegenerative or neuroprotective changes induced by these agents will be assessed using fluorescent indicators of cell viability, and immunocytochemical markers of the dendritic cytoskeleton, calcium-binding proteins and glutamate receptors.
Fluorescent systems operate on the basis that a chemically selective membrane containing a fluorescent indicator can provide an analytical signal caused by the change of the structure of the membrane due to the binding events.
In order to confirm that the Reichardt-Hassenstain model accurately describes the directional selectivity in the retina, the study was conducted using optical recordings of free cytosolic calcium levels after loading a fluorescent indicator dye into the fly tangential cells.
Although we fished with small fluorescent indicators stuck on our lines - the training wheels of short-line nymphing - the slightest pull from a submerged rock or weed was generally enough to prompt us to rip our rods skyward, as in one of those television fishing shows in which the angler hooks a huge marlin.