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After an additional wash, cells were fixed with 1% paraformaldehyde for flow cytometry analysis.
Further, the Strep-Tactin backbone has a fluorescent label to allow flow cytometry analysis.
Flow cytometry analysis identifies low-stage, low-grade tumors at high risk of recurrence by virtue of their aneuploid histograms.
RHW also participated in the conception as well as real-time PCR and flow cytometry analyses.
The increase seen in the frequency of TUNEL-positive germ cells in MAA-treated mice corroborates the results obtained from flow cytometry analysis.
Flow Cytometry Analysis For isolation and detection of peptide-specific T cells, patient PBMC samples were stained and analyzed by fluorescence-activated cell sorting (FACS) as previously described [2].
Apoptosis assay DT40 cells were treated for 24 h and flow cytometry analyses of apoptotic cells was performed using the TUNEL in situ cell death detection kit (Roche Molecular Biochemicals).
The results of the flow cytometry analysis showed similar changes in the rate of cellular proliferation as the BrdUrd method (Table III) but, these changs were of smaller magnitude indicating the lower sensitivity of this method.
In a first attempt to characterise Β 1 integrin cell membrane expression, flow cytometry analysis was performed with antibodies against various α chains of the Β 1 family of integrins after pretreatment with DMJ or DNJ.