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Unique among mycobacteria in its requirement for hemin or ferric ammonium citrate for growth.
Mix the Ferric Ammonium Citrate and Tartaric Acid solutions well together.
Careful QC is employed during manufacturing to ensure that the ferric ammonium citrate yields a consistent reproducible response to the various test cultures.
For example, if it desired to determine the number of iron related bacteria (IRB), ferric ammonium citrate may be included in the medium.
FAC solution: suspend ferric ammonium citrate in 10.0 mL of distilled water and filter sterilize.
Acriflavin (0.25% in distilled water) Ferric ammonium citrate (5.0% in distilled water) Filter sterilize the solutions.
It involves exposing materials which have been treated with a solution of potassium ferricyanide and ferric ammonium citrate to a UV light source such as the sun.
In a typical procedure, equal volumes of an 8.1% (w/v) solution of potassium ferricyanide and a 20% solution of ferric ammonium citrate are mixed.
The earlier Geritol liquid formulation was advertised as "twice the iron in a pound of calf's liver," and daily doses contained about 50-100 milligrams of iron as ferric ammonium citrate.
Media have to be supplemented with 0.4% haemoglobin or 60 uM hemin (factor X) or 15 mg/ml ferric ammonium citrate respectively, but not with FeCl or catalase.
Deliquescent salts include calcium chloride, magnesium chloride, zinc chloride, potassium carbonate, potassium phosphate, carnallite, ferric ammonium citrate, potassium hydroxide, ferric chloride, and sodium hydroxide.
Using this qualitative assay we have shown that all species of Listeria are able to reduce ferric iron when presented as ferric ammonium citrate whereas most other species of Gram-positive and Gram-negative bacteria are not.
A sector containing XLD (a selective media for Salmonella comprised of xylose, lysine, sodium desoxycholate, sodium thiosulfate, and ferric ammonium citrate) (Difco, Detroit, MI) was also included as an additional phenotypic test.