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Araldite is commonly used as an embedding medium for electron microscopy.
If the dielectric constant for the embedding medium is varied, the resonant frequency can be shifted.
(v) Because of its low viscosity Spurr resin has been found satisfactory as an embedding medium.
JB-4 (Polysciences), a water-soluble resin, has been found to be the optimal embedding medium.
The first half was frozen in liquid nitrogen in OCT embedding medium.
Brain tissue was placed on cryostat cork discs using a small amount of embedding medium (OCT compound, Miles).
Tissue samples were fixed in formalin, or were immediately frozen at -80 C in optimal cutting temperature embedding medium (OCT, Sakura).
Paraformaldehyde-fixed tissues were washed in a 15% solution of sucrose in PBS, placed in Tissue-Tek embedding medium, and stored in liquid nitrogen.
Polyacrylamide gel (PAG) had been known as a potential embedding medium for sectioning tissues as early as 1964, and two independent groups employed PAG in electrophoresis in 1959.
Another sample of the specimen was mounted in spurr's resin (an embedding medium used in electron microscopy) and sectioned with a diamond knife; the resulting sections were examined with transmission electron microscopy (TEM).
TUNEL Randomly selected hearts from control and cardiomyopathic turkeypoults were rapidly removed, flushed with PBS and infused with atissue embedding medium (Tissue-Tek, OCT Compound, Miles Inc., Elkhart,IN) and frozen in liquid nitrogen.
These analyses have shown that many commercial reagents, including embedding medium, contain alumino-silicate contaminants in low concentrations (p.p.m.), present either as a fine suspension of particles or in solution, when they crystallize out as the solvents evaporate; they may also preferentially locate on the sulphate and phosphate moieties present in the plaques.