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Using this procedure it was no longer necessary to destain the gel.
As the dye stained the polyacrylamide gel as well as the protein, to visualise the protein bands they needed to destain the gel which they did electrophoretically.
Ponceau S is the more common of the two, due to its higher sensitivity and water solubility, the latter making it easier to subsequently destain and probe the membrane, as described below.
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