cryostat section

Cryostat sections about 5 m thick were prepared and stained with methyl green for examination under a light microscope.

Briefly, cryostat sections were picked up on poly-L-lysone-coated slides and dried at 37 C for 2 h.

Cryostat sections (6 m) were cut and EGF distribution was studied immunohistochemically.

Cryostat sections (20 microns) from spinal cord and brain were cut and stored in phosphate buffer.

Connective tissue was demonstrated in routine and cryostat sections stained with trichrome and picrosirius methods.

Immunocytochemistry Antibody stainings were performed on cryostat sections of Calliphora erythrocephala .

Immunohistochemical processing Cryostat sections (14 microns) from the major pelvic ganglia were cut and thaw-mounted on gelatinized slides.

Histological diagnosis of all samples was performed using H&E-stained cryostat sections (JWR).

Cryostat sections were prepared to confirm suitability for profiling (Figure 1Aand 1B); only viable-appearing tissues were processed.

PCNA can be demonstrated by using monoclonal antibodies, usually requiring cryostat sections or specially prepared histological material.

Briefly, cryostat sections were fixed with acetone, dried, and rinsed in phosphate buffered saline (PBS).

This observation is consistent with a lack of LHR immunoreactivity in rat kidney cryostat sections reported previously [ 22 ] .

For frozen tissues, 5 μm cryostat sections (Leica Microsystems, Heidelberger, Germany) were briefly fixed in acetone.

Cryostat sections of rat brain were manipulated to deactivate biochemical guidance cues while preserving haptotactic cues and were then used as substrata for cultured neurons.

Acid phosphatase activity was used to detect lysosomal degradation in smooth muscle, and acetylcholinesterase staining was used to identify nerve fibre distribution in cryostat sections.

Deparaffinised and cryostat sections of the gastric biopsy specimens were evaluated for the presence of various antigens using the immunoalkaline phosphatase method (APAAP).

Immunohistochemical staining with the α-ALS serum also failed to show immunoreactivity in brain cryostat sections from Calliphora in contrast to positive controls made in Drosophila (data not shown).

Histochemistry To determine a character of muscle fibers (type II or type I) exhibiting changes in p27 expression, adjacent cryostat sections were stained for actomyosin adenosinetriphosphatase (ATPase).

Orthogonal cryostat sections of 20 μm were cut onto Superfrost slides (Fisher), and the edge of the section containing the outer nuclear layer was hand dissected using a microscalpel and immediately frozen.

Cryostat sections of 5 were dried overnight at room temperature and fixed in carbon tetrachloride at 4 C for 10 minutes, followed by extensive washing with phosphate buffered saline (pH 7.2) before staining.

Serial coronal cryostat sections (20 μm thick) were hybridized overnight at 55 C and washed at 57 C. Slides were exposed to Kodak Biomax film for 6 days at room temperature.

This limitation can be overcome by culturing neurons on cryostat sections where both the success and orientation of neurite growth on white matter have been shown to depend on the geometry of the underlying fiber tract [ 22].

Cryostat sections and cells were tested by indirect immunoperoxidase assays using rabbit anti-mouse antibody P260 Dakopatts (Hamburg, Germany) and the substrate 3-amino-9-ethylcarbazol Sigma (Deisenhofen, Germany) as an indicator system as described elsewhere.

Pathological changes either in the neural plexus or in the smooth muscle coats were not evident in routine or cryostat sections, or on histochemical staining, in the seven subjects from whom full thickness bowel samples were available for further analysis.

Cryostat sections, 5 m thick, were cut, mounted on poly-L-lysine coated microscope slides, air-dried, postfixed in 4% paraformaldehyde in phosphate buffered soline, rinsed, dehydrated through graded ethanol baths, and stored at -20 C with desiccant, for up to three months.