cryoprotectant

In this species the different levels of cryoprotectant can be used to distinguish between morphologies.

It survives being frozen by producing a cryoprotectant in its tissues.

Like the frogs, it was a glucose, or sugar, that acted as the cryoprotectant.

The cryoprotectant had worked, preserving and protecting him.

Large, representative samples of each population are frozen with glycerol as a cryoprotectant at 500-generation (75 day) intervals.

In the laboratory it is a common precipitant and cryoprotectant in protein crystallography.

Those solutes that do, such as dimethyl sulfoxide, a common cryoprotectant, are often toxic in intense concentration.

The glucose inside the cell acts as a cryoprotectant, a type of antifreeze, preserving the cell until thawed.

In this way, the cryoprotectant prevents actual freezing, and the solution maintains some flexibility in a glassy phase.

Work is continuing towards achieving whole-body vitrification, which is limited by the ability to fully circulate the cryoprotectant throughout the body.

The cells are incubated in a glycerol solution which acts as a cryoprotectant ("antifreeze") within the cells.

Dimethyl sulfoxide (DMSO) is also regarded as a conventional cryoprotectant.

A cryoprotectant is a substance that is used to protect biological tissue from freezing damage (i.e. that due to ice formation).

Arctic frogs use glucose, but Arctic salamanders create glycerol in their livers for use as a cryoprotectant.

DMSO may also be used as a cryoprotectant, added to cell media to reduce ice formation and thereby prevent cell death during the freezing process.

Thus, as the cryoprotectant replaces the water molecules, the biological material retains its native physiological structure and function, although they are no longer immersed in an aqueous environment.

As research in the 1990s revealed in greater detail the damaging effects of freezing, there was a trend to use higher concentrations of glycerol cryoprotectant to prevent freezing injury.

When they are thawed, it is done progressively, with the embryos put in a series of solutions to wash off the cryoprotectant and allow the normal water concentration of the embryos to return.

Although polyols such as sorbitol, mannitol, and ethylene glycol can also be found, glycerol is by far the most common cryoprotectant and can be equivalent to 20% of the total body mass.

The first human client received the vitrification mixture in the summer of 2005 using a new procedure in which the head was vitrified while still attached to the body, which was frozen without any cryoprotectant.

A controlled rate cooling process, allowing biological samples to equilibrate to optimal physical parameters osmotically in a cryoprotectant (a form of anti-freeze) before cooling in a predetermined, controlled way proved necessary.

It is also used as a cryoprotectant where the glycerol is dissolved in water to reduce damage by ice crystals to laboratory organisms that are stored in frozen solutions, such as bacteria, nematodes, and mammalian embryos.