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This gingival crevicular fluid is analyzed for a variety of factors, including prostaglandin, a hormone known to contribute to tissue and bone loss.
During mastication (chewing), the resultant compression forces acting on the periodontal ligament cause the simulated release of gingival crevicular fluid.
In addition to acting as a buffer, saliva is also a main source of nutrients for the thousands of bacteria (note: gingival crevicular fluid is also a nutrient source but a smaller one ).
The early lesion displays acute exudative inflammation; exudative components and crevicular lymphocytes reach their maximum levels between days 6-12 after plaque accumulates and gingival inflammation commences with the quantity of crevicular fluid being proportional to the size of the reaction site within the underlying connective tissue.