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In the 1930s he discovered countercurrent distribution, which was important in breaking down large chemicals.
About 490 000 hypothalamic fragments were submitted to alcohol–chloroform extraction, countercurrent distribution, ion-exchange chromatography, gel filtration, and partition chromatography.
Products were separated into unconjugated and "conjugated" (water-soluble) fractions by countercurrent distribution (CCD) using an ether-water partition.
Partitioning cells in a dextran polyethylene glycol aqueous two-phase system (countercurrent distribution, CCD) is a sensitive method for learning about cell surface membrane properties and for subfractionating cell populations.
Evolving from Craig's Countercurrent Distribution (CCD), the most widely used term and abbreviation is CounterCurrent Chromatography or CCC, in particular when using hydrodynamic CCC instruments.