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The Corrugator supercilii muscles can pull the eyebrows inwards and down, forming a frown.
The corrugator supercilii muscle, also known as the 'frowning' muscle, draws the brow down into a frown, and therefore is the best test for negative, unpleasant emotional response.
Havas and colleagues asked subjects to read emotional (angry, sad, happy) sentences before and two weeks after Botox injections in the corrugator supercilii muscle used in frowning.
The musculature of the forehead comprises the Occipitofrontalis, Procerus, and Corrugator supercilii muscles, all of which are controlled by the temporal branch of the facial nerve.
The Occipitofrontalis muscles produce the transverse wrinkles across the width of the forehead, and the Corrugator supercilii muscles produce vertical wrinkles between the eyebrows above the nose.
In a functional neuroimaging study, Andreas Hennenlotter and colleagues asked participants to perform a facial expression imitation task in an fMRI scanner before and two weeks after receiving botox injections in the corrugator supercilii muscle used in frowning.