confluency

Wells were selected based on cell confluency for expansion and further analysis.

Upon confluency of the well, the cells were trypsinized and expanded.

These results showed that the cell culture maintained satisfactory growth until confluency had been reached.

When they are very numerous, superimposition may give the impression of confluency, but this does not seem to occur in reality.

Ultramicroextensions were quite frequent between cells and on the surface of cells grown at confluency.

Briefly, COS cells were grown to 50% confluency on gelatin-coated glass cover slips.

For determination of newly synthesized collagenous proteins, fibroblasts were plated in 12-well plates and grown to visual confluency.

For adherent cells cell density is normally measured in terms of confluency, the percentage of the growth surface covered by cells.

Three days after reaching confluency (at about one week in culture), about half of the surface of the cells was covered with ultramicroextensions (Figure 1, D,E,F).

When cell cultures reached 40-60% confluency (usually, on the next day), growth medium was replaced with 2 ml of fresh medium containing variable concentrations of IFNg.

Cultured SVEC endothelial cells seeded in 24 wells were transfected at 70% confluency with SuperFect (Qiagen).

For preparation of donors in G0/G1 phase, the cells were allowed to grow to confluency by culturing for 6 days and a single cell suspension was prepared by standard trypsinization.

It is "kind of a wonderful confluency of celebrities and audience identification with them," said Nova Lanktree, director of the Burns Sports Celebrity Service, a Chicago consulting firm.

Quantitative pulse-chase analysis of fibrillin-1 protein Primary fibroblast cultures from patients and control individuals were grown to confluency and then metabolically labeled with 35S-cysteine as described [ 17 18 ] .

The dog gall bladder epithelial cells have similarities to bovine in a number of biological characteristics (time to reach confluency, division rates, etc) but the method we report in this paper is simpler and quicker.

Cell treatment and the immunoprecipitation (IP) of the δ-OR or the EGFR C6-δOR or HEK-δOR cells were not treated until they reached 80-90% confluency.

Trypsinization is often done to permit passage of the cells to a new container, observation for experimentation, or reduction of the degree of confluency in the flask by removal of a percentage of the cells.

Briefly, 2 μg 3TP-Luc together with 0.3 μg β-galactosidase expression plasmid were transfected into cells grown to 70-80% confluency in 6-well plates using LipofectAMINE reagent (Invitrogen) following the manufacturers protocol.

Gall bladder epithelial cells grew successfully and visible clusters of cells were present by day two, confluency being reached at 8 to 10 days in collagen coated plates and 12 to 14 days in uncoated plates.

After 2-4 days of subculturing, the cells (approximately 50% of confluency) were cultured in the medium supplemented with 0.3 μg/ml nocodazole (Sigma), a microtubule polymerization inhibitor, for 10 h to induce G2/M phase arrest.

Material and Methods Neutrophil isolation and cell culture Human umbilical vein endothelial cell (EC) cultures were purchased (Clontech, Palo Alto, CA) and maintained at 30-80% confluency to ensure high viability.

These cell types were all maintained in DMEM with 10% fetal calf serum with 1% antibiotic/ antimycotic, and seeded onto 24-well tissue culture plates at approximately 20,000 cells/cm 2; cultures were used immediately after reaching confluency.

This was assessed by two independent investigators by observing (a) the cell appearance and their ability to attach the plate in the first 24 hours and (b) the length of time the culture required to reach confluency from the same starting number of cells per well.

In cell culture biology, confluency is the term commonly used as a measure of the number of the cells in a cell culture dish or a flask, and refers to the coverage of the dish or the flask by the cells.

For example, 100 percent confluency means the dish is completely covered by the cells, and therefore no more room left for the cells to grow; whereas 50 percent confluency means roughly half of the dish is covered and there is still room for cells to grow.