cDNA clone

Only the shorter transcript has been isolated in cDNA clones.

However, several independent partial cDNA clones (see Figure 2) contain the same 5' nucleotide.

Gene models based on incompletely assembled cDNA clones were marked 'incomplete'.

The cDNA clones were sequenced from both ends using standard forward and reverse primers.

Information on protein similarities, gene expression, cDNA clones, and genomic location is included with each entry.

This work witnessed the isolation of the first cDNA clones encoding collagen and fibronectin.

Part of the protein was sequenced and that sequence was used to allow isolation of a cDNA clone.

This is consistent with GenBank record, which indicates that the cDNA clone was also isolated from brain tissue.

The yeast two-hybrid system and colony hybridization screens used in this study indicated that a monkey cDNA clone (Fig.

First, we intend to increase the number of genes represented in this set of fully vetted cDNA clones using a combination of experimental approaches.

Data processing and assembly cDNA clone data management relied on custom scripts and an Informix database.

However, promoter prediction is complicated by the fact that most cDNA clones do not extend to the TSS.

The positive cDNA clones were plaque purified and the Bluescript phagemid (Stratagene) rescued.

The reported full-length mouse and human cDNA clones encode proteins of 2052 and 2098 amino acid residues, respectively.

ESTs are single-pass, partial sequences of cDNA clones from a large number of disease and normal tissue libraries.

Partial cDNA clones are shown by dotted lines at the 5' end; translation termination codons are indicated by an asterisk.

The complete nucleotide sequence of the longest cDNA clone 14-6, isolated from the original BL-29 library, was determined and is shown in Fig. 3 a.

In this report, we describe isolation of silk gland EF-1α cDNA clones and nucleotide sequence of a clone.

A 1.4 kb human cDNA clone, described previously and designated λPt2 (10), was used for Southern hybridization and library screening.

Moreover, EST-containing sets often contain 5' and 3' reads from the same cDNA clone, but these sequences do not always overlap.

This step converts the small adaptor ligated RNAs into cDNA clones used in the sequencing reaction.

The probes were prepared from PCR amplification of the cDNA clones used for printing the Lymphochip.

Multiple cDNA clones were identified by screening the U251 library with a bacterial lysate absorbed polyclonal antiserum against human tenascin.

Furthermore, none of the new SM1 type cDNA clones hybridized to a 5' end-labelled primer encoding the visceral-specifc isoform.

The hybridization of synthetic oligonucleotides to arrayed cDNAs yields a fingerprint for each cDNA clone.