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When you overwhelm the capacity of the buffer salt the pH will change.
Buffering salts like ammonium salts predominated the 20th century.
For simplicity I have written only singly charged cations and anions and have considered only one source of buffering salt.
Another significant factor may be the difference in charge between the buffer salts; phosphate is anionic whereas Tris is cationic and zwitterionic.
Before using the balance to measure say a 100 g of buffer salt, I have carry out a check weight using a calibrated 500 gram check weight.
One "liquid oven" concept developed for molten salt cooled, solid fueled reactors, employs a separate buffer salt pool where the entire primary loop is suspended in.
A simple and effective method is by maintaining a pH buffer level by adding suitable pH buffer salt to counter the acidification.
The destaining solution for CBB contains usually the buffer salt ammonium bicarbonate (NHHCO) and a fraction of 30%-50% organic solvent (mostly acetonitrile).
Isolated cells were washed three times in Hank's buffered salt solution (Flow Laboratories, Irvine, Scotland), then suspended in culture medium and adjusted to a final concentration of 2x10 cells/ml.
They are grown in Medium 199 (a solution containing buffered salt, vitamins, amino acids, fetal bovine serum) with SPGA(sucrose, phosphate, glutamate, human serum albumin) and neomycin.
Nevertheless, a separate desalting step is usually not necessary as in previous steps of sample preparation e.g. in-gel digestion of proteins, the selection of appropriate buffer salts prevents the occurrence of this problem.
The BCA protein assay method maintains the high sensitivity and low variability among proteins associated with the Lowry protein assay, while providing increased tolerance to the presence of non-ionic detergents and buffer salts.
The cells were detached with 0.2 mg/ml trypsin (Worthington Biochemical Corporation, NJ, USA) and 10 mM EDTA in calcium- and magnesium-free Hank's buffered salt solution and resuspended in L-15.
Because of the high heat capacity and considerable density of the buffer salt, the buffer salt not only prevents fuel salt freezing, but also participates in the passive decay heat cooling system, provides radiation shielding, and reduces deadweight stresses on primary loop components.