biuret reaction

Franz Hofmeister made the proposal in the morning, based on his observations of the biuret reaction in proteins.

This is achieved by the chelation of bicinchoninic acid with the cuprous ion formed by the biuret reaction.

Hofmeister also argued for peptide bonds based on the biuret reaction observed with all proteins but never with free amino acids.

The Biuret reaction can be used to assess the concentration of proteins because peptide bonds occur with the same frequency per amino acid in the peptide.