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Except for the rare autotrophic bacteria, nothing lived in this realm.
The most common design uses two different basins: one catering to the autotrophic bacteria and the second to the heterotrophic bacteria.
Autotrophic bacteria, or bacteria that derives its energy making its own food by oxidation, like the Nitrobacters species, rather than feeding on plants or other organisms.
Carboxysomes are intracellular structures found in many autotrophic bacteria such as Cyanobacteria, Knallgasbacteria, Nitroso- and Nitrobacteria.
Chemical and microbial analyses both indicate that a rare subglacial ecosystem of autotrophic bacteria developed that metabolizes sulfate and ferric ions.
Typical autotrophic bacteria are phototrophic cyanobacteria, green sulfur-bacteria and some purple bacteria, but also many chemolithotrophic species, such as nitrifying or sulfur-oxidising bacteria.
Many autotrophic bacteria and archaea fix carbon via the reductive acetyl CoA pathway, the 3-hydroxypropionate cycle, or the reverse Krebs cycle.
Given CODH's role in CO fixation identified in diverse autotrophic bacteria and archaea, it is common in the biochemistry literature for the reductive mechanism to be inferred as the "direct reverse" of the oxidative mechanism by the "principal of microreversibility."