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An incision was made over the dorsal aspect of the neck and the overlying muscles were retracted to expose the atlanto-occipital membrane.
Posterior Atlanto-Occipital Membrane: genetic traits can sometimes result in ossification, turning the groove into an foramen.
An intrathecal catheter (PE 10) was introduced through a small slit in the atlanto-occipital membrane and positioned over the L6/S1 spinal cord segments [ 33 ] .
At the end of the experiment, 5 μl of fast-green (1%) was infused through the cannula while observing the CSF though a small slit in the atlanto-occipital membrane.