amoebocyte

To each tube, 25 l of reconstituted limulus amoebocyte lysate was added, before incubation for 35 minutes at 37 C.

Amoebocyte extract from horseshoe crab blood is mixed with a sample suspected of endotoxin contamination, and a reaction is observed if endotoxins are present.

The blood contains a chemical called Limulus amoebocyte lysate (LAL) that can be used to detect pathogens and their endotoxins.

From this basic research, a reagent, Limulus amoebocyte lysate (LAL), was developed that can detect minute amounts of bacterial toxins.

The presence of lipopolysaccharide biological activity was demonstrated by the Limulus amoebocyte lysate test, pyrogenicity in rabbits, complement interaction inhibiting the erythrocyte lysis, and chicken-embryo lethality.

The plasma was stored at -80 C until assayed for endotoxin using a modified limulus amoebocyte lysate assay (Coatest Endotoxin, Kabi Diagnostica, Molndal, Sweden).