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The alpha-1 fraction does not disappear in alpha 1-antitrypsin deficiency, however, because other proteins, including alpha-lipoprotein and alpha-1 acid glycoprotein, also migrate there.
ICG has the ability to bind 98% to plasma proteins - 80% to globulins and 20% to alpha-lipoprotein and albumin - and thus, in comparison with fluorescein as a marker, has a lower leakage (slower emergence of dye from the vessels, extravasally).