Transwell

Paul Transwell is the fourth I’ve written about (so imagine how many have written to me).

Transwell says he “hates” minimalism.

O - Daylight hours C - Mandy Transwell, address and telephone number as above.

Migration assay Cell migration was assessed in 24-well chemotaxis chambers fitted with 5.0 μm transwell membranes.

The transwell membranes were removed after 90 min of incubation at 37 C, and migrated cells in the lower chamber were harvested by pipetting.

Control for chemotaxis was performed by adding 1000 ng/ml cCAF to both wells of the Transwell unit.

Consistent with the transwell experiments, conditioned medium induces and maintains CRCs, which is accompanied by a concomitant increase of cellular telomerase activity.

In brief, MGBE were grown on Vitrogen-coated Transwell inserts suspended above human gallbladder myo-fibroblast feeder cells.

For cultured monolayers, these chambers use the Snapwell device - a 2-piece Transwell with 12 mm diameter x 0.4 um pore membrane mounted in a detachable ring.

Because a microfluidic lung-on-a-chip can more exactly reproduce the mechanical properties of a living human lung, its physiological responses will be quicker and more accurate than a Transwell culture system.

To test whether the cCAF-induced effect is due to chemotaxis, we repeated the transwell assay and compared cell migration when cCAF is added to both wells.

Caco-2 cells were grown as confluent monolayers on transwell filters and tight-junction formation was confirmed by the inability of inulin to diffuse from the apical to the basolateral surface.

Transwell assay for migration 1 x 10 6cells in 100 μl 199 medium containing 2% serum were plated on the underside of transwell membranes by inverting the insert.

Over-expression of GnT-V in these cells increased the cleavage and shedding of PTPkappa ectodomain and increased migration of WiDr cells in transwell assays.

The pressure-driven dynamic motion behind the stretching of the membrane, also described as a cyclic mechanical strain (valued at approximately 10%), significantly increases the rate of nanoparticle translocation across the porous membrane, when compared to a static version of this device, and to a Transwell culture system.

Because tissues treated with cCAF have more FN and TN at the time that these cells are entering the wound and chemokines are well known for their chemoattractant properties [ 34 35 ] , we tested whether cCAF stimulates chemotaxis of fibroblasts using transwell assays.