Sanger sequencing

The licensing terms include the exclusive rights to commercialize the technology combined with Sanger sequencing.

The device consists of three functional units, each corresponding to the Sanger sequencing steps.

Even still, 1.96 million indels have been identified through Sanger sequencing that do not overlap with other databases.

There are several sequencing platforms available including the classical Sanger sequencing.

However, de novo genome assembly by Sanger sequencing is extremely expensive and time consuming.

Recent studies use shotgun Sanger sequencing or pyrosequencing to recover the sequences of the reads.

These mutations were identified through Sanger sequencing.

This technique offers a number of advantages over traditional sequencing methods such as Sanger sequencing.

These read lengths are significantly shorter than the typical Sanger sequencing read length of 750 bp.

When used for detection of genetic and genomic changes, jumping clones require validation by Sanger sequencing.

The mutation was identified by whole-exome sequencing and confirmed by Sanger sequencing.

Using this method, up to 17 gigabases can be sequenced at once, as opposed to limited ranges for Sanger sequencing of only about 1 kilobase.

They employed whole genome sequencing, along with Sanger sequencing to identify the gene that was mutated in these families.

Another limitation of this system is the short read length compared to other sequencing methods such as Sanger sequencing or pyrosequencing.

When RRBS was first developed, Sanger sequencing was initially used.

These technologies are vastly superior to Sanger sequencing due to the high volume of data and the relatively short time it takes to sequence a whole genome.

This new sequencing method generated reads much shorter than those of Sanger sequencing: initially about 100 bases, now 400-500 bases.

Instead, the metadata associated with it can be converted into a more informative format-for Sanger sequencing, the results are a trace file, which looks like this:

Sequencher 5.1 encompasses the ability to perform Sanger Sequencing and Next Generation Sequencing.

From a computational perspective, microfluidic Sanger sequencing is still the most effective way to sequence and assemble genomes for which no reference genome sequence exists.

The classical shotgun sequencing was based on the Sanger sequencing method: this was the most advanced technique for sequencing genomes from about 1995-2005.

More recently, Sanger sequencing has been supplanted by "Next-Gen" sequencing methods, especially for large-scale, automated genome analyses.

Nevertheless, the implementation of Sanger sequencing for decoding DNA-encoded chemical libraries in high-throughput fashion was the first to be described.

Recent studies use "shotgun" Sanger sequencing or massively parallel pyrosequencing to get largely unbiased samples of all genes from all the members of the sampled communities.

Molecular techniques, such as Sanger sequencing, resulting in a much broader scope but highlighting the more abundant species present (Heidelberg et al., 2010)(Pedros-Alio, 2007).