Maxam-Gilbert sequencing

An automated Maxam-Gilbert sequencing protocol was developed in 1994.

Maxam-Gilbert sequencing reactions were performed as described [ 37 ] .

Maxam-Gilbert sequencing is no longer in widespread use, having been supplanted by next-generation sequencing methods.

Maxam-Gilbert sequencing is a method of DNA sequencing developed by Allan Maxam and Walter Gilbert in 1976-1977.

Maxam-Gilbert sequencing requires radioactive labeling at one 5' end of the DNA and purification of the DNA fragment to be sequenced.

Maxam-Gilbert sequencing was the first widely-adopted method for DNA sequencing, and, along with the Sanger dideoxy method, represents the first generation of DNA sequencing methods.

In addition, a G+A Maxam-Gilbert sequencing reaction (35) was carried out on 4ng of the particular end-labelled probe used in each DNase I footprinting experiment to provide a sequencing ladder for orientation of the footprint.

However, with the improvement of the chain-termination method (see below), Maxam-Gilbert sequencing has fallen out of favour due to its technical complexity prohibiting its use in standard molecular biology kits, extensive use of hazardous chemicals, and difficulties with scale-up.