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They look like EC cells but do not contain 5-HT.
By aggregating the cells into embryonic body, EC cells can also process differentiation.
The electrolytic processes in the EC cell are controlled electrically and with no moving parts, thus requiring less maintenance.
Layer V EC cells have strong recurrent excitatory synapses much like CA3 layers in the hippocampus and when provoked are capable of burst activity.
Competition with the same binding sites in F9 EC cell extracts is shown in tracks 2 and 3; tracks 1 to 5 were taken from the same experiment.
Interactions between integrin αβ and MMP-2 on the EC cell surface may be necessary for MMP-2 activity during angiogenesis.
DP-1 cDNA clones were frequently rearranged and the nucleotide sequence shown is from cDNAs from an F9 EC cell library.
The effect was not as marked as in F9 EC cell extracts, probably because some of the DP-1 polypeptide loses its N-terminal region during purification (data not shown).
Peptide 5 antisense oligonucleotide was used to synthesize cDNA from F9 EC cell RNA which was then used in a PCR with both peptide 6 and 5 primers.
Polypeptides in affinity-purified DRTF1/E2F (about 5μg from about 5x10 10 F9 EC cells) were separated by SDS-polyacrylamide gel electrophoresis and stained with Coomassie blue (track 2); track 1 shows M r standards.
Although similarities in morphology and differentiating potential (pluripotency) led to the use of EC cells as the in vitro model for early mouse development, EC cells harbor genetic mutations and often abnormal karyotypes that accumulated during the development of the teratocarcinoma.