CDNA library

Hybridization results were further analyzed by considering the cDNA library of origin.

Clones were found in a mouse thymus cDNA library, however.

The cv-1 cDNA library was amplified and stored as per the manufacturer's instructions.

Similarly, tissue specific cDNA libraries can be produced.

The substrate is a total cDNA library.

Seven cDNA libraries are available online.

There are 2 variations of the experimental procedure for a given cDNA library (see Fig.

This cDNA library was then differentially screened using the method of Leonard et al.

Once selected, stocks of the bacteria are created which can later be grown and sequenced to compile the cDNA library.

Isoforms are readily described and discovered by microarray studies and cDNA libraries.

It was identified during a full transcriptome screen of the C. elegans cDNA library.

The depth of sequencing and special manipulation are not consistent across cDNA libraries, which could also influence the sensitivity of the analysis.

Other common ways of selecting genes for arrays may also violate this assumption, including selection based on matches in specific cDNA libraries.

Applications of cDNA libraries include:

It was successfully cloned from a cDNA library in 1995 revealing that, after processing, it is 477 nucleotides long.

Reverse transcriptase is used also to create cDNA libraries from mRNA.

It also provides cDNA library screening and quantitative PCR.

Colony hybridizations were performed on E. coli colonies containing the monkey cv-1 cDNA library.

We used chromosome 19 as the model to test this hypothesis by comparing the cDNA library profiles of spatially adjacent paralogous genes.

The primary aim of this parameter is to identify and eliminate unrelated sequences that were physically linked by a cDNA library construction artifact.

A similar observation was recently reported for the expression of genes isolated specifically from blastoderm-specific cDNA libraries [ 7].

This suggests that their identification by sequencing miRNA cDNA libraries would have been unlikely.

To prepare a cDNA library, the first step is to isolate the total mRNA from the cell type of interest.

Comparing human and rat serine dehydratase using a cDNA library was identical except for a 36 amino acid residue.

It was found by screening a peptide containing the C-terminal domain of dystrophin against cDNA libraries.