"pair" nach Englisch mit Beispielen - Kollokationen-Wörterbuch Englisch
- Thus, many sequences (up to 40) can be amplified and quantified using just a single primer pair.
- Specific products obtained with each primer pair from various organs and in several independent experiments were verified by sequencing.
- We could design primer pairs for 56 out of 83 unique fragments (see Additional File 1).
- NetPrimer can be used to determine the best primer pairs for a given set of experimental conditions.
- This requires at least one primer (for a given primer pair) to have the specified number of mismatches to unintended targets.
- Increasing this number can increase the chance of finding a specific primer pair but the process will take longer.
- The annealing temperatures for each primer pair are listed in Table 1.
- From 56 primer pairs, 19 could detect a polymorphism among 24 peanut genotypes (see Table 1).
- All positive clones were confirmed by PCR with appropriate primer pairs.
- However, such multiplex amplifications have the inherent problem that each primer pair has its own specific conditions of hybridization and thus control is not perfect.
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