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- In this respect, bisulfite sequencing has much higher resolution (down to a single CpG site; single nucleotide level).
- The parallel structure differs substantially from the fold-back structure at the individual nucleotide level.
- All clones displayed 98-99% identity at the nucleotide level, and the differences between sequences of various strains did not exceed the diversity within one strain.
- This G protein subunit activates a taste phosphodiesterase and decreases cyclic nucleotide levels.
- However unlike traditional restriction based methods, bisulfite sequencing provided resolution on a nucleotide level (Eads 2000; Laird 2010).
- However, at the nucleotide level they are only 86% identical.
- One lithography step is needed per base type; thus, a total of four steps is required per nucleotide level.
- Several kinds of formula sold abroad contain nucleotide levels that are comparable to human milk, but only one is sold in this country.
- This confirmed that these junctions were unaltered at the nucleotide level (data not shown), indicating that integration had indeed occurred by homologous recombination.
- The use of next-generation sequencing technology to study the transcriptome at the nucleotide level is known as RNA-Seq.
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